aav dj egfp Search Results


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Cell Biolabs Inc aav/dj-dio-ef1α-cheriff-egfp
a, Confocal images of a slice co-electroporated with <t>CheRiff</t> and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.
Aav/Dj Dio Ef1α Cheriff Egfp, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Biolabs Inc aav-dj-egfp
a, Confocal images of a slice co-electroporated with <t>CheRiff</t> and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.
Aav Dj Egfp, supplied by Cell Biolabs Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Biolabs aav9/dj-cag-egfp
a, Confocal images of a slice co-electroporated with <t>CheRiff</t> and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.
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N/A
eGFP-AV01 AAVPrime™ Purified AAV Particles, serotype AAV-DJ/8, 025 µl(Old Cat # AA401)
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eGFP-AV01 AAVPrime™ Purified AAV Particles, serotype AAV-DJ, 025 µl(Old Cat AA201)
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Adeno-Associated Virus-DJ (AAV-DJ) is a synthetic serotype made from eight different wild-type AAV serotypes (AAV2, 4, 5, 8, 9, avian, bovine, and goat AAV) using DNA shuffling. These modifications give the AAV-DJ serotype improved transduction
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Image Search Results


a, Confocal images of a slice co-electroporated with CheRiff and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.

Journal: Nature methods

Article Title: Fast, in vivo voltage imaging using a red fluorescent indicator

doi: 10.1038/s41592-018-0188-7

Figure Lengend Snippet: a, Confocal images of a slice co-electroporated with CheRiff and VARNAM (n = 5 fields of view). Single and double arrows in the merged image indicate neurons expressing one or both constructs, respectively. b,c, Optical and electrical spikes induced by 10 Hz (b) and 50 Hz (c) blue-light pulses (vertical blue lines) (0.5 ms, 0.5–1 mW mm−2). Arrow in c indicates spike failure. Gray box indicates interval shown at an expanded time scale. *Blue-light artifact (1 mW mm−2). d, Fluorescence responses to 400 ms blue-light illumination (blue steps) at increasing intensities (0.4–2.4 mW mm−2). e, Confocal images of a fixed slice from PV-Cre+ mouse showing cell-type-specific VARNAM and CheRiff expression (n = 7 fields-of-view). f,g, Mean optical and electrical IPSPs evoked by (f) blue-light pulses (2.5 mW mm−2) at depolarized (left) and resting potentials (right) and (g) longer trains of pulses at depolarized potentials. n = 15 trials, each. Shading denotes s.e.m.

Article Snippet: We generated a recombinant AAV encoding floxed CheRiff-EGFP (AAV/DJ-DIO-EF1α-CheRiff-EGFP) using the AAV-DJ Helper Free Packaging System (Cell Biolabs, Inc), following pre-established procedures 48 .

Techniques: Expressing, Construct, Fluorescence